Arp2/3 mediates early endosome dynamics necessary for the maintenance of PAR asymmetry in Caenorhabditis elegans

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie 01 - Movie for Figure 3B cropped time series Example of early endosome moving back towards plasma membrane in association with actin in embryo expressing moe-GFP and RAB-5-mCherry.
  • Movie 02 - Movie for Figure 3C cropped time series Example of early endosome fission in association with actin in embryo expressing moe-GFP and RAB-5-mCherry.
  • Movie 03 - Movie for Figure 4Aii cropped time series Example of GFP-PAR-6 vesicle-like structure moving into cytoplasm in association with RAB-5-mCherry positive early endosomes.
  • Movie 04 - Movie for Figure 4B - control Control embryo expressing GFP-PAR-6 and RAB-5-mCherry showing few cytoplasmic aggregates.
  • Movie 05 - Movie for Figure 4B - arx-2 RNAi arx-2 RNAi-treated embryo expressing GFP-PAR-6 and RAB-5-mCherry showing cytoplasmic aggregates of both.