Electron Transfer in Subunit NuoI (TYKY) of Escherichia coli NADH:Quinone Oxidoreductase (NDH-1)

Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 (.pdf, 32 KB) - Coomassie Blue-stained SDS-PAGE gel from a sample of purified E. coli. complex I
• Supplemental Figure 2 (.pdf, 34 KB) - Similarity of the deduced amino acid sequences among the E. coli NuoI subunit and its homologues from various organisms
• Supplemental Figure 3 (.pdf, 124 KB) - NADH dehydrogenase activity staining (A) and immunoblotting (B) of BN-PAGE gels of membrane preparations from E. coli.
• Supplemental Figure 4 (.pdf, 63 KB) - EPR spectra of the purified E. coli complex I from wild type and NuoI mutant E107A. The top panel (A)
• Supplemental Table 1 (.pdf, 22 KB) - Primers used for the mutagenesis of E. coli nuoI Gene.
• Supplemental Table 2 (.pdf, 20 KB) - Purification of NDH-1 complex from E. coli MC4100.
• Supplemental Table 3 (.pdf, 25 KB) - Effects of NuoI mutations on production of superoxide radicals coupled to dNADH oxidase