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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: J Neurosci Methods. 2012 Apr 10;207(2):137–147. doi: 10.1016/j.jneumeth.2012.04.003

Figure 2. Drebrin clusters reliably label spines and correlate with spine morphology.

Figure 2

(A) DIV12 neurons were transfected with a plasmid expressing GFP. Four days after transfection (DIV16), neurons were fixed, permeabilized, and stained wtih Drebrin (red) and MAP2 (blue). Colocalization (yellow) of GFP and drebrin on spines in merged images demonstrates that drebrin clusters localize on spines (see circled region). Scale bar is 10μm. (B) Significant direct correlation between drebrin cluster fluorescence intensity and area covered by GFP in spine heads (r2 = 0.8899, p< 0.0001).