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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: J Neurosci Methods. 2012 Apr 10;207(2):137–147. doi: 10.1016/j.jneumeth.2012.04.003

Figure 7. Densities of mature and immature spines.

Figure 7

Densities of NR1-only, GluR2-only, and NR1+GluR2 immunoreactive spines per 20μm dendrite segments from DIV10-26 neurons. Triple-colocalization of drebrin, NR1 and GluR2 was used to measure the density of NR1+GluR2 immunoreactive spines. The density of NR1-only (or GluR2-only) spines was obtained by subtracting NR1+GluR2 spine density from the total spine-localized NR1 (or GluR2) density. Data are presented as ± 95% confidence interval; p<0.05: * for NR1, # for GluR2, & for NR1+GluR2, n.s, no significance.