Fig. 6.
Titration of phosphorylated Erk levels in 10A.B2.TGFb cells by using U0126. (A) 10A.B2.TGFb cells were seeded in transwell migration chambers in the presence of AP1510 and various doses of the Mek inhibitor U0126. After 18 h, migration was quantified as described. (B) Cells prepared for the migration assay were plated in parallel, lysed after 18 h, and pErk1/2 and total Erk levels were analyzed by Western blot.