Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Supplemental materials and methods, geographic maps showing the location of Lake Kasin in southern Russia and the exact sampling position at the lake about 50 m away from the water-covered area (Fig. S1), most probable number counts of Fe(III)-reducing and anaerobic Fe(II)-oxidizing microorganisms from the top 10 cm of different salt lake sediments in mineral medium with 5 M or 0.5 M NaCl (Fig. S2), DGGE gels of PCR amplicons of partial 16S rRNA gene fragments from enrichments from Lake Kasin sediment (0 to 10 cm) that reduce Fe(III) over several transfers (Fig. S3), sequences of qPCR primers applied in this study (Table S1), thermal profiles and standards used for qPCR in this study (Table S2), MÖssbauer parameters for Lake Kasin sediment spectra of the Fe-rich sediment layer at 1- to 3-cm depth (Table S3), and phylogenetic affiliation of the 16S rRNA gene sequences from the archaeal and bacterial clone libraries from Lake Kasin sediment (Table S4).
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