Correlative Time-Resolved Fluorescence Microscopy To Assess Antibiotic Diffusion-Reaction in Biofilms

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Movie sequence of BODIPY-vancomycin penetration inside S. aureus ATCC 6538 biofilm obtained by time-lapse imaging. It shows the evolution of fluorescence intensity in the biofilm 5 μm above the glass surface. Real time (4 min) is indicated in the lower lefthand corner.
  QuickTime Movie file, 1.3MB.
• Supplemental file 2 - A section representation of ATCC 6538 (a) and ATCC 27217 (b) S. aureus biofilms stained with a live-dead probe and ATCC 15442 P. aeruginosa biofilm (c) stained with Syto9. For P. aeruginosa biofilm, FCS measurements were taken in two zones, inside (zone 1) and outside (zone 2) a mushroom structure.
  Zipped PDF file, 154K.