Supplemental Data
Figure S1: Stereospecific reverse effect on accelerated raft fluidity induced by FA2H depletion. Figure S2: Amplified images of figure 3A. Figure S3: Pref-1 mRNA levels in FA2H knockdown adipocytes and in adipocytes treated with 2-OH PA enantiomers. Figure S4: MRM chromatograms obtained from the analysis of C16:0 2-OHCer in adipocyte extracts and authentic standard C12:0 2-OHCer spiked in the lipid extracts. Figure S5: Regulation of C16:0 Cer and HexCer by FA2H and 2-OH PA enantiomers. Figure S6. A proposed model for differential interaction of sphingolipids containing (R)-2-OH FA or (S)-2-OH FA.
Files in this Data Supplement:
- Supplemental Figures 1-6 - Figure S1: Stereospecific reverse effect on accelerated raft fluidity induced by FA2H depletion. Figure S2: Amplified images of figure 3A. Figure S3: Pref-1 mRNA levels in FA2H knockdown adipocytes and in adipocytes treated with 2-OH PA enantiomers. Figure S4: MRM chromatograms obtained from the analysis of C16:0 2-OHCer in adipocyte extracts and authentic standard C12:0 2-OHCer spiked in the lipid extracts. Figure S5: Regulation of C16:0 Cer and HexCer by FA2H and 2-OH PA enantiomers. Figure S6. A proposed model for differential interaction of sphingolipids containing (R)-2-OH FA or (S)-2-OH FA.