Figure S1: Q-Q and Manhattan Plots for association with ln CRP in HUFS 

Table S1: Top 50 SNPs associated with lnCRP in HUFS (adjusted for age, sex, BMI, and T2D)

SNP	Chr.	Positiona	Type	Ancestral allele	Closest gene	Distance to gene	P-value	
rs16827466	1	159649700	Intergenic	C	RP11-419N10.3*	25073	4.31E-11	
rs726640	1	159655518	Intergenic	G	RP11-419N10.3*	19255	4.99E-11	
rs7531832	1	159654216	Intergenic	A	RP11-419N10.3*	20557	5.94E-11	
rs16842525	1	159664624	Intergenic	T	RP11-419N10.3*	10149	6.79E-10	
rs1341666	1	159667059	Intergenic	C	RP11-419N10.3*	7714	6.84E-10	
rs3093058	1	159685315	Upstream	T	CRP	-936	7.55E-10	
rs6667499	1	159677654	Upstream	G	RP11-419N10.3*	-2257	9.35E-10	
rs9628671	1	159687972	Upstream	C	CRP	-3593	1.34E-09	
rs16842520	1	159657850	Intergenic	C	RP11-419N10.3*	16923	1.79E-09	
rs12239267	1	159670928	Downstream	C	RP11-419N10.3*	3845	2.43E-09	
rs16827462	1	159627891	Intergenic	G	RP11-419N10.3*	46882	9.75E-08	
rs6781222	3	115125869	Intergenic	G	RP11-190P13.2	121675	9.54E-07	
rs1909574	3	115143941	Intergenic	C	RP11-190P13.2	139747	1.07E-06	
rs1392261	3	115144685	Intergenic	G	RP11-190P13.2	140491	1.07E-06	
rs10508679	10	24793880	Intronic	A	KIAA1217	0	1.43E-06	
rs25913	5	108993934	Intergenic	C	MAN2A1	-31133	1.57E-06	
rs9867344	3	45360872	Intergenic	G	U3.20	-63760	1.70E-06	
rs13018903	2	142608963	Intronic	A	LRP1B	0	1.71E-06	
rs13090809	3	115134513	Intergenic	G	RP11-190P13.2	130319	1.75E-06	
rs7899691	10	24792171	Intronic	G	KIAA1217	0	1.76E-06	
rs9809772	3	45335495	Intergenic	T	U3.20	-38383	2.01E-06	
rs854215	3	45379597	Intergenic	A	LARS2	-50401	2.17E-06	
rs6931563	6	19656557	Intergenic	T	U6.212	-13797	2.24E-06	
rs17756735	12	107942604	Intronic	G	BTBD11	0	3.64E-06	
rs31610	5	108997418	Intergenic	A	MAN2A1	-27649	4.12E-06	
rs25915	5	108992687	Intergenic	A	MAN2A1	-32380	4.17E-06	
rs8093291	18	77701294	Intronic	G	PQLC1	0	4.43E-06	
rs1366108_a	5	-9	N/A	N/A	N/A	-9	4.50E-06	
rs9290659	3	178344908	Intronic	T	KCNMB2	0	4.71E-06	
rs13431583	2	142594611	Intronic	T	LRP1B	0	5.38E-06	
rs13385804	2	142617164	Intronic	A	LRP1B	0	5.59E-06	
rs11946653	4	130951432	Intergenic	A	RP11-422J15.1	74909	5.73E-06	
rs7756468	6	19657782	Intergenic	G	U6.212	-15022	5.83E-06	
rs7756553	6	19658108	Intergenic	A	U6.212	-15348	5.83E-06	
rs1366108	5	68114936	Intergenic	G	CTC-340D7.1	13476	5.93E-06	
rs2913278	5	68115600	Intergenic	G	CTC-340D7.1	12812	5.95E-06	
rs1427905	5	68118222	Intergenic	A	CTC-340D7.1	10190	5.95E-06	
rs7557872	2	126679292	Intergenic	G	AC097499.2	98400	6.23E-06	

(S1 continued)
rs7190999	16	13956228	Intergenic	G	ERCC4	-57786	6.45E-06	
rs12096077	1	238620372	Intergenic	G	RP11-371I1.2	23312	6.72E-06	
rs7152651	14	91043744	Intronic	C	TTC7B	0	6.86E-06	
rs9366306	6	19667184	Intergenic	T	RP3-471C18.2	22872	7.09E-06	
rs6456256	6	19651463	Intergenic	C	U6.212	-8703	7.21E-06	
rs16847370	2	142612131	Intronic	T	LRP1B	0	7.24E-06	
rs10864064	1	213877776	Intergenic	T	RP11-323I1.1	-26983	7.38E-06	
rs1794336	16	13950359	Intergenic	C	ERCC4	-63655	8.06E-06	
rs551042	1	170132339	Intronic	C	METTL11B	0	8.49E-06	
rs4655461	1	213864971	Intergenic	A	RP11-323I1.1	-39788	9.52E-06	
rs7586153	2	142616136	Intronic	T	LRP1B	0	9.73E-06	
rs644796	1	181436054	Intronic	C	CACNA1E	0	1.01E-05	
* RP11-419N10.3: CRP pseudogene1 (CRPP1)
a Position of the SNPs were derived from dbSNP  genome build 37.2

Table S2: Top 50 SNPs associated with lnCRP in HUFS (adjusted for age, sex, BMI, T2D, smoking and hypertension)
SNP	Chr.	Positiona	Type	Ancestral allele	Closest gene	Distance to gene	P-value	
rs16827466	1	159649700	Intergenic	C	RP11-419N10.3*	25073	2.97E-11	
rs726640	1	159655518	Intergenic	G	RP11-419N10.3*	19255	3.27E-11	
rs7531832	1	159654216	Intergenic	A	RP11-419N10.3*	20557	3.95E-11	
rs3093058	1	159685315	Upstream	T	CRP	-936	4.28E-10	
rs16842525	1	159664624	Intergenic	T	RP11-419N10.3*	10149	4.72E-10	
rs1341666	1	159667059	Intergenic	C	RP11-419N10.3*	7714	4.99E-10	
rs6667499	1	159677654	Upstream	G	RP11-419N10.3*	-2257	6.23E-10	
rs9628671	1	159687972	Upstream	C	CRP	-3593	8.30E-10	
rs16842520	1	159657850	Intergenic	C	RP11-419N10.3*	16923	1.14E-09	
rs12239267	1	159670928	Downstream	C	RP11-419N10.3*	3845	2.09E-09	
rs16827462	1	159627891	Intergenic	G	RP11-419N10.3*	46882	8.66E-08	
rs6781222	3	115125869	Intergenic	G	RP11-190P13.2	121675	8.72E-07	
rs1909574	3	115143941	Intergenic	C	RP11-190P13.2	139747	9.81E-07	
rs1392261	3	115144685	Intergenic	G	RP11-190P13.2	140491	9.81E-07	
rs10508679	10	24793880	Intronic	A	KIAA1217	0	1.14E-06	
rs7899691	10	24792171	Intronic	G	KIAA1217	0	1.32E-06	
rs25913	5	108993934	Intergenic	C	MAN2A1	-31133	1.40E-06	
rs13090809	3	115134513	Intergenic	G	RP11-190P13.2	130319	1.52E-06	
rs9867344	3	45360872	Intergenic	G	U3.20	-63760	1.62E-06	
rs6931563	6	19656557	Intergenic	T	U6.212	-13797	1.79E-06	
rs9809772	3	45335495	Intergenic	T	U3.20	-38383	1.79E-06	
rs13018903	2	142608963	Intronic	A	LRP1B	0	1.97E-06	
rs25915	5	108992687	Intergenic	A	MAN2A1	-32380	3.71E-06	
rs9290659	3	178344908	Intronic	T	KCNMB2	0	3.81E-06	
rs644796	1	181436054	Intronic	C	CACNA1E	0	3.97E-06	
rs854215	3	45379597	Intergenic	A	LARS2	-50401	4.56E-06	
rs7756468	6	19657782	Intergenic	G	U6.212	-15022	4.61E-06	
rs7756553	6	19658108	Intergenic	A	U6.212	-15348	4.61E-06	
rs31610	5	108997418	Intergenic	A	MAN2A1	-27649	4.81E-06	
rs11946653	4	130951432	Intergenic	A	RP11-422J15.1	74909	5.39E-06	
rs7190999	16	13956228	Intergenic	G	ERCC4	-57786	5.67E-06	
rs13431583	2	142594611	Intronic	T	LRP1B	0	5.81E-06	
rs13385804	2	142617164	Intronic	A	LRP1B	0	6.20E-06	
rs6456256	6	19651463	Intergenic	C	U6.212	-8703	6.32E-06	
rs9366306	6	19667184	Intergenic	T	RP3-471C18.2	22872	6.75E-06	
rs2899193	22	33109382	Intronic	C	SYN3	0	6.91E-06	
rs8093291	18	77701294	Intronic	G	PQLC1	0	7.04E-06	

(S2 continued)
rs4655461	1	213864971	Intergenic	A	RP11-323I1.1	-39788	7.08E-06	
rs10864064	1	213877776	Intergenic	T	RP11-323I1.1	-26983	7.09E-06	
rs12096077	1	238620372	Intergenic	G	RP11-371I1.2	23312	8.57E-06	
rs16847370	2	142612131	Intronic	T	LRP1B	0	8.58E-06	
rs17756735	12	107942604	Intronic	G	BTBD11	0	8.61E-06	
rs551042	1	170132339	Intronic	C	METTL11B	0	8.91E-06	
rs1366108_a	5	-9	N/A	N/A	N/A	-9	9.53E-06	
rs845741	5	109001791	Intergenic	A	MAN2A1	-23276	1.01E-05	
rs7586153	2	142616136	Intronic	T	LRP1B	0	1.09E-05	
rs1794336	16	13950359	Intergenic	C	ERCC4	-63655	1.11E-05	
rs9832697	3	178261186	Intronic	C	KCNMB2	0	1.13E-05	
rs7557872	2	126679292	Intergenic	G	AC097499.2	98400	1.17E-05	
rs7152651	14	91043744	Intronic	C	TTC7B	0	1.20E-05	
* RP11-419N10.3: CRP pseudogene1 (CRPP1)
a Position of the SNPs were derived from dbSNP genome build 37.2

Table S3.  Selected SNPs nominally associated with lnCRP in HUFS
SNP	Chr.	Positiona	Type	Ancestral allele	closest gene	distance to gene	P-value	
rs2027471	1	159689388	INTERGENIC	T	CRP	-5009	2.3x10-05	
rs1341665	1	159691559	INTERGENIC	G	CRP	-7180	2.3x10-05	
rs7553007	1	159698549	INTERGENIC	G	CRP	-14170	2.9x10-05	
rs13018903	2	142608963	INTRONIC	A	LRP1B	0	1.7x10-06	
rs13431583	2	142594611	INTRONIC	T	LRP1B	0	5.4x10-06	
rs13385804	2	142617164	INTRONIC	A	LRP1B	0	5.6x10-06	
rs16847370	2	142612131	INTRONIC	T	LRP1B	0	7.2x10-06	
rs7586153	2	142616136	INTRONIC	T	LRP1B	0	9.7x10-06	
rs16847412	2	142618914	INTRONIC	T	LRP1B	0	1.2x10-05	
rs13430058	2	142615596	INTRONIC	G	LRP1B	0	2.98x10-05	
rs25913	5	108993934	INTERGENIC	C	MAN2A1	-31222	1.6x10-06	
rs31610	5	108997418	INTERGENIC	A	MAN2A1	-27738	4.1x10-06	
rs25915	5	108992687	INTERGENIC	A	MAN2A1	-32469	4.2x10-06	
rs845741	5	109001791	INTERGENIC	A	MAN2A1	-23365	1.1x10-05	
rs31614	5	108995505	INTERGENIC	T	MAN2A1	-29651	1.9x10-05	
rs25914	5	108993021	INTERGENIC	T	MAN2A1	-32135	2.3x10-05	
rs7190999	16	13956228	INTERGENIC	G	ERCC4	-57786	6.5x10-06	
rs1794336	16	13950359	INTERGENIC	C	ERCC4	-63655	8.1x10-06	
rs231124	16	13966418	INTERGENIC	C	ERCC4	-47596	1.2x10-05	
rs857164	16	13958817	INTERGENIC	G	ERCC4	-55197	1.3x10-05	
rs231125	16	13966386	INTERGENIC	C	ERCC4	-47628	1.7x10-05	

a Position of the SNPs were derived from dbSNP genome build 37.2

Table S4. Conditional association analysis using rs3093058 as covariate in the linear regression
SNP	r2	type	 Original association p-value	Association p-value when conditioned on rs3093058	gene	
rs16827466	0.68	INTERGENIC	4.3E-11	0.02	RP11-419N10.3*	
rs726640	0.83	INTERGENIC	5.0E-11	0.04	RP11-419N10.3*	
rs7531832	0.83	INTERGENIC	5.9E-11	0.04	RP11-419N10.3*	
rs16842525	0.90	INTERGENIC	6.8E-10	0.34	RP11-419N10.3*	
rs1341666	0.90	INTERGENIC	6.8E-10	0.34	RP11-419N10.3*	
rs3093058	1	UPSTREAM	7.6E-10	-	CRP	
rs6667499	0.98	UPSTREAM	9.4E-10	-	RP11-419N10.3*	
rs9628671	0.99	UPSTREAM	1.3E-9	-	CRP	
rs16842520	0.89	INTERGENIC	1.21E-09	0.34	RP11-419N10.3*	
rs12239267	0.81	DOWNSTREAM	2.4E-09	0.27	RP11-419N10.3*	
rs16827462	0.55	INTERGENIC	9.8E-08	0.45	RP11-419N10.3*	
rs2027471	0.07	INTERGENIC	2.3E-05	0.01	CRP	
rs1341665	0.07	INTERGENIC	2.3E-05	0.01	CRP
	
rs7553007	0.06
	INTERGENIC
	2.9E-05
	0.02
	CRP
	
* RP11-419N10.3: CRP pseudogene1 (CRPP1)

Table S5. Anthropometric and clinical characteristics of the West African replication sample: AADM

Parameters	Male	Female	P-Value	

N	174	312		

Age (years)	51.4±8.0	48.3±8.2	8.52E-05	

BMI (Kg/m2)	25.5±5.1	29.1±6.3	6.75E-11	

Type 2 diabetic (%)	58.1	50.6	0.11	

Smoking (%)	11	0.65	<.0001	

Hypertensive (%)	54.6	49.7	0.3	

SBP (mm Hg)	138.5±23.3	134.5±22.0	0.07	

DBP (mm Hg)	85.8±13.5	81.8±13.03	0.001	

Cholesterol (mg/dl)	180.6±52.1	187.5±56.8	0.19	

HDL-cholesterol(mg/dl)	39.2±29.5	45.2±29.3	0.03	

LDL-cholesterol (mg/dl)	119.9±59.1	124.52±66.7	0.45	

Triglycerides (mg/dl)	107.7±96.0	88.9±54.1	0.02	
Hs-CRP 
Mean (mg/dl)
Geometric mean (mg/dl)	
0.43±0.8
0.17	
0.5±1.1
0.21	0.45	
Values are mean ± standard deviation except for hypertension, Type 2 diabetes and smoking which are frequencies. The mean were compared by student’s t-test and the frequencies by chi-square.