Modular TRAPP Complexes Regulate Intracellular Protein Trafficking Through Multiple Ypt/Rab GTPases in Saccharomyces cerevisiae

Supporting Information for Zou et al, 2012

Supporting Information

• Supporting Information - Figures S1-S5, File S1, and Table S1 (PDF, 3.9 MB)
• Figure S1 - GST-Bet5, but not GST-Trs85, complex purified from yeast lysates can act as a GEF for Ypt31 and Ypt32 (PDF, 838 KB)
• Figure S2 - A temperature-sensitive growth phenotype is linked to over-expression of GFP-Snc1 in trs85∆ mutant cells (PDF, 1.1 MB)
• Figure S3 - Accumulation of intra-cellular GFP-Snc1 in ypt1ts and trs85∆ts mutant cells is different from its accumulation in ypt31∆/32ts and trs130ts (PDF, 895 KB)
• Figure S4 - Ypt1 suppresses the growth defect of ypt1ts, whereas Ypt31 suppresses the growth defect of ypt31∆/32ts (PDF, 575 KB)
• Figure S5 - Ypt32 suppresses the growth defect and GFP-Snc1 phenotype of ypt31∆/32ts and trs130ts (PDF, 687 KB)
• File S1 - Supporting Materials and Methods (PDF, 75 KB)
• Table S1 - Yeast strains and plasmids used in this study (PDF, 93 KB)