Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast

Supplemental Materials

This article contains the following supporting material:

• Movie01 - Video 1. Time lapse analysis of Chs2 GFP and Dbf2 mCherry localization during cytokinesis (related to Figure 1D). 2 min interval. Scale bar, 2 �m.
• Movie02 - Video 2. Time lapse analysis of Chs2 GFP, Chs2 S217A GFP, and Chs2 S217D GFP localization with respect to septin hourglass splitting (related to Figure 4A). 1 min interval. Scale bar, 2�m.
• Movie03 - Video 3. Time lapse analysis of Chs2 GFP, Chs2 S217A S225A GFP, and Chs2 S217D S225D GFP localization with respect to actomyosin ring constriction (related to Figure 4D). 1 min interval. Scale bar, 2�m.
• Movie04 - Video 4. FRAP analysis of Chs2 GFP, Chs2 S217A GFP, and Chs2 S217D GFP during cytokinesis (related to Figure 5A). 20 sec interval. Scale bar, 2�m.
• Movie05 - Video 5. Time lapse analysis of Chs2 GFP localization with respect to septin hourglass splitting in wild type cells and dbf2 Ts dbf20 cells carrying pGAL1 SIC1s (related to Figure 6A). 2 min interval. Scale bar, 2�m.