Video 3.
Accumulation of Atg9 at the Golgi apparatus in cells depleted of Sec7. Video associated with Fig. S2 A. ATG9-2×GFP DRS2-TagRFPT cells and ATG9-2×GFP DRS2-TagRFPT SEC7-AID cells were treated with rapamycin for 30 min and then with 500 µg/ml IAA for 1 h. Images were analyzed by time-lapse microscopy at 32 ms/frame using an inverted microscope (IX71). In ATG9-2×GFP DRS2-TagRFPT SEC7-AID cells treated with IAA, Atg9-2×GFP formed immobile structures. WT, wild type.