Video 10.
3D animation of the telophase shown in Fig. 5 (A and B) and Video 9. (blue) DAPI; (red) actin; (green) mNumb. Fast confocal microscopy was used to collect the image. An acquisition typically consisted of 60 planes × 4 channels with a z step of 0.5 µm and a pixel size of 141 nm. For creating 3D rotations of a volume comprising this cell, an image stack of a volume encompassing it was created using the Imaris 3D cropping function. Viewing 3D rotations of the F-actin signal allows visualizing the BP linking one sister with the basal lamina. Well visible is that the daughter cell, in which mNumb is asymmetrically segregated, is also the one that is connected to the basal lamina by the BP.