Synthetic Motility and Cell Shape Defects Associated with Deletions of Flotillin/Reggie Paralogs in Bacillus subtilis and Interplay of These Proteins with NfeD Proteins

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Fig S1, domain structures of FloT and FloA

  Fig. S2, fluorescence microscopy images of Bacillus subtilis cells expressing ComFA-mCherry as a marker for DNA uptake machinery

  Table S1, oligonucleotides

  Legends to Movies S1 to S7

  PDF, 89K

• Supplemental file 2 -

  Movie S1, TIRF-M time lapse microscopy of cells expressing FloT-YFP from the original gene locus

  AVI, 1.7M

• Supplemental file 3 -

  Movie S2, TIRF-M time lapse microscopy of cells expressing FloT-YFP from the original gene locus

  AVI, 338K

• Supplemental file 4 -

  Movie S3, TIRF-M time lapse microscopy of cells expressing FloA-YFP from the original gene locus

  AVI, 378K

• Supplemental file 5 -

  Movie S4, epifluorescence time lapse microscopy of cells expressing FloA-YFP from the original gene locus

  AVI, 589K

• Supplemental file 6 -

  Movie S5, TIRF-M time lapse microscopy of cells expressing FloT-YFP from the original gene locus, 60 min after addition of FCCP

  AVI, 187K

• Supplemental file 7 -

  Movie S6, TIRF-M time lapse microscopy of cells expressing NfeD2-YFP from the original gene locus

  AVI, 66K

• Supplemental file 8 -

  Movie S7, three-dimensional reconstruction of Z-stacks taken through an S2 cell expressing FloT-YFP

  AVI, 4.5M