A Listeria monocytogenes RNA Helicase Essential for Growth and Ribosomal Maturation at Low Temperatures Uses Its C Terminus for Appropriate Interaction with the Ribosome

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Fig. S1, multiple-sequence alignment of Lmo1722 and Lmo1722_ΔCT from Listeria monocytogenes and YfmL from Bacillus subtilis

  Fig. S2, growth of L. monocytogenes wild type and its isogenic Δlmo1722 derivative on BHI plates at the indicated temperatures

  Fig. S3, Western blot analysis examining expression levels of Lmo1722 in the indicated L. monocytogenes strains

  Fig. S4, growth of L. monocytogenes strains at different temperatures

  Fig. S5, outline of the primer extension analysis of the wild-type strain carrying pIMK3 and the Δlmo1722/pIMK3 strain as shown in Fig. 4C

  Table S1, oligonucleotides

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