The Wood Rot Ascomycete Xylaria polymorpha Produces a Novel GH78 Glycoside Hydrolase That Exhibits α-l-Rhamnosidase and Feruloyl Esterase Activities and Releases Hydroxycinnamic Acids from Lignocelluloses

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Supplemental materials and methods.
  PDF file, 21K.
• Supplemental file 2 - Effects of temperature and pH on the activity of XpoGH78 and stability of purified XpoGH78 over time (8 hours) at different pH values and temperatures (Fig. S1).
  PDF file, 40K.
• Supplemental file 3 - Amino acid sequence of X. polymorpha hydrolase (Fig. S2).
  PDF file, 120K.
• Supplemental file 4 - Evolutionary relationship of eukaryotic and prokaryotic GH78 α-L-rhamnosidases of group I (Fig. S3).
  PDF file, 248K.
• Supplemental file 5 - Evolutionary relationship of eukaryotic and prokaryotic GH78 α-L-rhamnosidases of group II (Fig. S4).
  PDF file, 171K.
• Supplemental file 6 - Oligonucleotides used as primers for the identification of the XpoGH78 encoding gene (Table S1).
  PDF file, 22K.
• Supplemental file 7 - Results of a screening of different basidio- and ascomyceteous fungi for the production of feruloyl esterase (FAE) activity using an agar plate assay as well as maximum FAE activities detected with methyl ferulate for these fungi in solid-state cultures during 25 days of incubation (Table S2).
  PDF file, 31K.