Novel Phytases from Bifidobacterium pseudocatenulatum ATCC 27919 and Bifidobacterium longum subsp. infantis ATCC 15697

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Oligonucleotides used for cloning (Table S1).
    PDF file, 7K.
  • Supplemental file 2 - Refined multiple sequence alignment of PhypA from B. pseudocatenulatum ATCC 27919 (Fig. S1), phylogenetic analysis of phosphatases from different origins belonging to the branch 2 HAP group, including PhylA and PhypA from bifidobacteria (Fig. S2), and schematic representation of the PhylA and PhypA phytases and the truncated 6×(His)-tagged version lacking the signal peptide and the hydrophobic C-terminal resion, and SDS-PAGE analysis of purified 6×(His) truncated phytases (Fig. S3).
    PDF file, 368K.