Methane-Cycling Microbial Communities and Methane Emission in Natural and Restored Peatlands

Supplemental material

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  Detrended correspondence analysis (DCA) of vegetation and principal component analysis (PCA) of peat chemistry at depths of 0–7.5 cm and 7.5–15 cm of natural (N₁–N₃) and restored (R₁–_R3) peatlands (Fig. S1); phylogenetic tree of partial McrA sequences (133 aa) from peatland clones and reference sequences (Fig. S2); phylogenetic tree of partial pmoA sequences (435 bp) from peatland DGGE bands and reference methanotroph sequences (Fig. S3); supplemental Materials and Method (vegetation and peat chemistry, quantitative PCR, statistical analyses, analysis of T-RFLP data, phylogenetic analysis); distribution of mcrA terminal fragments (T-RFs) (Table S1) and pmoA DGGE bands (Table S2) in analysis of methanogen communities in natural (N₁–_N3) and restored (R₁-_R3) peatlands.

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