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Detailed Materials and Methods; SDS-PAGE of samples from resting-cell experiments involving incubation of the A. tertiaricarbonis L108 wild-type strain and knockout mutant strain (ΔmdpJ) K24 in the presence of TBA(Fig. S1); degradation of TBA in resting-cell experiments (Fig. S2); identification of 2-butanol metabolites 2-butanone and 3-buten-2-one (Fig. S3); GC-MS analysis of 2-butanol metabolites (Fig. S4); GC-FID and GC-MS analyses of 3-methyl-2-butanol metabolites (Fig. S5) and of 3-pentanol metabolites (Fig. S6); substrates and corresponding products of MdpJ-catalyzed hydroxylation and desaturation of tertiary and secondary alcohols (Fig. S7).
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