Files in this Data Supplement:
Purification of recombinant CDH IIA and CDH IIB produced by P. pastoris (Table S1), substrate specificities of CDH IIA and CDH IIB for various carbohydrates measured with the indicated electron acceptors at pH 5.0 or 6.0 (Table S2), substrate inhibition values of N. crassa CDH IIA and CDH IIB for cellobiose and lactose in the presence of 300 μM DCIP as electron acceptor for pH values between 4.0 and 7.0 (Table S3), codon-optimized nucleotide sequence and translated polypeptide of N. crassa GH61-3 NCU02916 for heterologous expression in P. pastoris (Fig. S1), supernatant of fermentation samples of N. crassa GH61-3 NCU02916 applied on an SDS-PAGE and developed with Coomassie blue (Fig. S2), SDS-PAGE (Fig. S3), inhibition of N. crassa CDH IIA (Fig. S4) and CDH IIB (Fig. S5) by cellobiose and lactose in the presence of DCIP as electron acceptor at pH values between 4.0 and 7.0, and inhibition of the cyt c reducing activity of CDH IIA and CDH IIB at pH 6.0 by 10 μM GH61-3, 10 μM BSA, and 10 μM copper sulfate (Fig. S6).
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