Fig. 5.
Southern blot of the genomic DNA of untransformed WT N. attenuata plants of the Utah genotype (WT; lane 1); two homozygous T3 independently transformed lines of the Utah genotype that had been transformed either with a construct containing 175 bp of the pi gene in an antisense orientation (AS–, AS-; lanes 2 and 3), or with an empty vector construct (C1, C2; lanes 4 and 5); digested with EcoRI and of untransformed plants of the Arizona (A; lane 6); and transformed plants of Arizona (S++; lane 7) digested with HindIII and hybridized with a radiolabeled probe from the nopaline synthase terminator of the vector plasmid pRESC 20 (Fig. 6).