Table 2. Absolute and relative mean differences in lifetime seed capsule production from pairs of developmentally synchronized plants from homozygous T3 independently transformed lines of a WT genotype of N. attenuata that had been transformed with constructs containing 175 bp of the pi gene in an antisense orientation (AS–, AS-), or an empty vector construct (C1, C2)

 

Competitors

Mean difference in capsule no.

P

% Mean difference in capsule no.

P

AS– vs, C1

33.7 ± 4.5

<0.0001

28.4 ± 2.3

<0.0001

AS–* vs. C1*

47.3 ± 7.3

<0.0001

48.6 ± 3.4

<0.0001

AS1– vs. C1*

64.6 ± 4.7

<0.0001

60.3 ± 4.1

<0.0001

AS- vs. C2

9.5 ± 1.8

0.0008

25.0 ± 4.0

0.0002

AS-* vs. C2*

10.6 ± 3.2

0.0009

41.1 ± 5.9

0.0004

AS- vs. C2*

14.6 ± 1.3

<0.0001

44.5 ± 2.7

<0.0001

Two plants were grown in the same pot in two different pair combinations (AS– vs. C1 and AS- vs. C2). Plants were treated either with 150 m g of MeJA in 20 m l of lanolin paste (*) to elicit JA-induced defenses or with 20 m l of pure lanolin paste; n = 10 per treatment and pair combination. The AS– vs. C1 pairs were grown under a minimum of 1,000-1,300 m mol·m-2·s-1 photosynthetic photon flux density (PPFD), and the AS- pairs were grown under a minimum of 800–900 m mol·m-2·s-1 PPFD. P values are from paired t test comparisons; relative values were arsine square root transformed before analysis.