Table 8.

ROS measurements^a

Strain and treatment	Mean fluorescence	SD	P valueb
SC5314, untreated	13.6	1.1
SC5314, 12 μg/ml DFD-VI-15	18.1	0.8	0.005*
SC5314, 24 μg/ml DFD-VI-15	19.6	1.2	0.003*
GOA31	24.9	1.2

^aThe mean fluorescence of DCFDA was compared between nontreated and treated (12 and 24 μg/ml of DFD-VI-15) cells of C. albicans SC5314 and GOA31. There was significantly higher fluorescence in treated strains than in untreated strains. These data show that there is a higher accumulation of intracellular ROS upon treatment with DFD-VI-15. Strain C. albicans GOA31, which is known to overproduce ROS following deletion of its GOA1, was used as a positive control (33).

^bP value compared to untreated SC5314 calculated by unmatched t test. *, statistically significant (P < 0.01).