Gbx2 Directly Restricts Otx2 Expression to Forebrain and Midbrain, Competing with Class III POU Factors

Supplemental material

Fig. S1 (EMSA of Lef1 and Otx2 binding to 157FM), S2 (EMSA of Brn1, Brn4, and Oct6 binding to X29), S3 (RT-PCR analysis of marker expression), S4 (Temporal changes of Otx1, et al., expression and of Wnt signaling), and Fig. S5 (Brn2, Oct6, and Gbx2 coding sequences and locations of miRNA target sequences) and Table S1 (Mass spectrometric analysis of proteins interacting with 157FM), S2 to S4 (Primers used for plasmid construction [S2] and RT-PCR [S3 and S4]), and S5 (Oligonucleotides used to construct miRNA expression plasmids)

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  • Supplemental file 1 -

    Fig. S1 (EMSA of Lef1 and Otx2 binding to 157FM), S2 (EMSA of Brn1, Brn4, and Oct6 binding to X29), S3 (RT-PCR analysis of marker expression), S4 (Temporal changes of Otx1, et al., expression and of Wnt signaling), and Fig. S5 (Brn2, Oct6, and Gbx2 coding sequences and locations of miRNA target sequences) and Table S1 (Mass spectrometric analysis of proteins interacting with 157FM), S2 to S4 (Primers used for plasmid construction [S2] and RT-PCR [S3 and S4]), and S5 (Oligonucleotides used to construct miRNA expression plasmids)

    PDF, 907KB