Files in this Data Supplement:
Movie 1. The centrosome localizes near the PAS during peripheral axon initiation. Movie of neuron shown in Fig. 3C labeled with TagRFP-CAAX in a GFP-Xcentrin mRNA-expressing embryo. Images were captured every minute. Yellow arrowhead indicates centrosome.
Movie 2. The centrosome moves to the PAS as the successful axon begins extension. Movie of neuron shown in Fig. 3E labeled with GFP-Zcentrin only. A neurite initiates and retracts on the left. The successful axon forms from the cell body and the centrosome moves to its PAS as it begins extension.
Movie 3. DN-CLIM causes centrosome mispositioning and aberrant axons. Movie of neuron shown in Fig. 5A labeled with TagRFP-CAAX in a GFP-Xcentrin mRNA- and DN-CLIM-expressing embryo. The centrosome (yellow arrowhead) is positioned more apically during ectopic apical neurite formation.