Video 5.
Interphase progression in CycA+B RNAi-treated embryos. Embryos expressing histone-RFP (red) were injected with dsRNA targeting CycA and CycB and subsequently with recombinant GFP-PCNA protein (green). Nuclei near the injection site are shown as they progress through interphase 13. Images were analyzed by time-lapse confocal microscopy using a spinning-disk confocal microscope (PerkinElmer). Frames were taken every 30 s. This video corresponds to Fig. 3 B. The 00:00 frame in Fig. 3 B corresponds to 14:11:45 in the video. Note that GFP-PCNA foci disappeared around 14:28:11; however, the nuclei did not enter mitosis until 14:33:44. Time is given in hours, minutes, and seconds.