Video 7.
Interphase progression in CycB+B3 RNAi-treated embryos. Embryos expressing histone-RFP (red) were injected with dsRNA targeting CycB and CycB3 and subsequently with recombinant GFP-PCNA protein (green). Nuclei near the injection site are shown as they progress through interphase 13. Images were analyzed by time-lapse confocal microscopy using a spinning-disk confocal microscope (PerkinElmer). Frames were taken every 30 s. This video corresponds to Fig. 3 D. The 00:00 frame in Fig. 3 D corresponds to 14:15:46 in the video. Note that GFP-PCNA foci disappeared around 14:28:51; however, the nuclei did not enter mitosis until 14:42:01. Time is given in hours, minutes, and seconds.