Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movies 1-4. Representative time-lapse movies illustrating the movement of GFP-EEA1 labeled structures (as in Figure 4C). Cos7 cells were cotransfected with (1) control, (2) p27, (3) Arp11, or (4) p150Glued siRNA. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie02 - Movies 1-4. Representative time-lapse movies illustrating the movement of GFP-EEA1 labeled structures (as in Figure 4C). Cos7 cells were cotransfected with (1) control, (2) p27, (3) Arp11, or (4) p150Glued siRNA. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie03 - Movies 1-4. Representative time-lapse movies illustrating the movement of GFP-EEA1 labeled structures (as in Figure 4C). Cos7 cells were cotransfected with (1) control, (2) p27, (3) Arp11, or (4) p150Glued siRNA. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie04 - Movies 1-4. Representative time-lapse movies illustrating the movement of GFP-EEA1 labeled structures (as in Figure 4C). Cos7 cells were cotransfected with (1) control, (2) p27, (3) Arp11, or (4) p150Glued siRNA. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie05 - Movies 5-8. Representative time-lapse movies illustrating the movement of recycling endosome (as in Figure 5E). Cos7 cells transfected with (5) control, (6) p27, (7) Arp11, or (8) p150Glued siRNA were labeled with Alexa 555-Tfn for 2 min, then imaged during a 10 to 15 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie06 - Movies 5-8. Representative time-lapse movies illustrating the movement of recycling endosome (as in Figure 5E). Cos7 cells transfected with (5) control, (6) p27, (7) Arp11, or (8) p150Glued siRNA were labeled with Alexa 555-Tfn for 2 min, then imaged during a 10 to 15 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie07 - Movies 5-8. Representative time-lapse movies illustrating the movement of recycling endosome (as in Figure 5E). Cos7 cells transfected with (5) control, (6) p27, (7) Arp11, or (8) p150Glued siRNA were labeled with Alexa 555-Tfn for 2 min, then imaged during a 10 to 15 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie08 - Movies 5-8. Representative time-lapse movies illustrating the movement of recycling endosome (as in Figure 5E). Cos7 cells transfected with (5) control, (6) p27, (7) Arp11, or (8) p150Glued siRNA were labeled with Alexa 555-Tfn for 2 min, then imaged during a 10 to 15 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 10X real time.
- Movie09 - Movies 9-12. Representative time-lapse movies illustrating the movement of late endosomes (as in Figure 6C). Cos7 cells transfected with (9) control, (10) p27, (11) Arp11, or (12) p150Glued siRNA were labeled with Alexa 555-α2M for 30 min, then imaged during a 90-120 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 6X real time.
- Movie10 - Movies 9-12. Representative time-lapse movies illustrating the movement of late endosomes (as in Figure 6C). Cos7 cells transfected with (9) control, (10) p27, (11) Arp11, or (12) p150Glued siRNA were labeled with Alexa 555-α2M for 30 min, then imaged during a 90-120 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 6X real time.
- Movie11 - Movies 9-12. Representative time-lapse movies illustrating the movement of late endosomes (as in Figure 6C). Cos7 cells transfected with (9) control, (10) p27, (11) Arp11, or (12) p150Glued siRNA were labeled with Alexa 555-α2M for 30 min, then imaged during a 90-120 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 6X real time.
- Movie12 - Movies 9-12. Representative time-lapse movies illustrating the movement of late endosomes (as in Figure 6C). Cos7 cells transfected with (9) control, (10) p27, (11) Arp11, or (12) p150Glued siRNA were labeled with Alexa 555-α2M for 30 min, then imaged during a 90-120 min chase interval. Movies were made in ImageJ with Sorensen normal compression. Playback is set at 6X real time.