Biochemical and pharmacological characterization of human α/β-hydrolase domain containing 6 (ABHD6) and 12 (ABHD12)

Supplemental Data

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• Supplementary Figures 1-5, Supplementary Table 1 - Supplementary Figure 1. Substrate profiles of hABHD6 and hABHD12 from four separate transfections. Supplementary Figure 2. Relative activity of hABHD6 and hABHD12 in HEK293 cell lysates and membrane preparations. Supplementary Figure 3. pH dependence of hABHD6 and hABHD12 activities. Supplementary Figure 4. Primary structure comparisons between human, mouse, and rat orthologs of ABHD6 and ABHD12. Supplementary Figure 5. Kinetic monitoring of glycerol output from 1(3)-AG and 2-AG by the endocannabinoid hydrolases hABHD6, hABHD12 and hMAGL. Supplementary Table 1. hABHD6 and hABHD12 possess no detectable fatty acid amide hydrolase (FAAH) activity.