RhoA activation during polarization and cytokinesis of the early Caenorhabditis elegans embryo is differentially dependent on NOP-1 and CYK-4

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie01 - Figure 1 movie 1 Time-lapse nomarski images of the first division of wild-type, nop-1(it142), and nop-1(RNAi) embryos. Times shown are relative to anaphase onset.
  • Movie02 - Figure 2 movie 1 Time-lapse nomarski images of wild-type, nop-1(it142) embryos, GFP::NOP-1, and nop-1(it142); GFP::NOP-1 embryos. Times shown are relative to anaphase onset.
  • Movie03 - Figure 2 movie 2 Time-lapse confocal images from embryos expressing GFP::NOP-1. Times shown from the beginning of each sequence.
  • Movie04 - Figure 3 movie 1 Time-lapse confocal images from control and nop-1(it142) or nop-1(RNAi) embryos expressing mCherry histone and (A) NMY-2::GFP, (B) GFP::ANI-1, and (C) GFP::Utrophin. Times shown are relative to anaphase onset.
  • Movie05 - Figure 3 movie 2 Time-lapse nomarski images of wild-type, nop-1(it142), rga-3/4(RNAi), nop-1(it142);rga-3/4(RNAi), ect-2(ax751ts), and ect-2(ax751ts);nop-1(RNAi). Times shown are relative to anaphase onset.
  • Movie06 - Figure 4 movie 1 Time-lapse nomarski images of wild-type, nop-1(it142), zen-4(RNAi), nop-1(it142); zen-4(RNAi), cyk-4(or749ts), and cyk-4(or749ts); nop-1(RNAi) embryos. Times shown are relative to anaphase onset.