Biosynthesis of the Polymannose Lipopolysaccharide O-antigens from Escherichia coli Serotypes O8 and O9a Requires a Unique Combination of Single- and Multiple-active Site Mannosyltransferases

Supplemental Data

Files in this Data Supplement:

• Supplemental Figs and Tables (.pdf, 1.9 MB) - Table S1: Primer sequences used for the production of recombinant plasmids and chromosomal mutants. Table S2: MS/MS confirmation of the products generated by WbdC^O9a and WbdCB^O9a using the synthetic GlcNAc-PP-C₁₃ acceptor. FIGURE S1: ¹H-¹³C gHSQC spectrum for the products synthesized by WbdA^O9a using Acceptor B. FIGURE S2: gCOSY and tROESY spectra for the products synthesized by WbdA^O9a using Acceptor B. FIGURE S3: ¹H-¹³C gHSQC spectrum for the products synthesized by WbdA^O8 using Acceptor A. FIGURE S4: gCOSY and tROESY spectra for the products synthesized by WbdA^O8 using Acceptor A.