Supplemental Data
Files in this Data Supplement:
- Supplemental movie 1 (.mpg, 1.9 MB) - Supplemental movie 1: immunofluorescence microscopy images (complete z-stack corresponding to Fig. 6A). HaCaT cells were treated with Alexa546-alpha-toxin (magenta) for 20 min, fixed, and stained for p-eIF2� (green). Note foci of labelled alpha-toxin in peripheral planes that extend into p-eIF2alpha-positive spots in deeper planes.
- Supplemental movie 2 (.mpg, 973 KB) - Supplemental movie 2: immunofluorescence microscopy images (complete z-stack) of HaCaT cells transfected with Flag-CReP, treated with Alexa546-alpha-toxin (magenta) for 20 min, fixed, and stained with antibodies against Flag (green). Note Alexa546-alpha-toxin accumulation in peripheral planes, juxtaposed to vesicle- and reticular structures in deeper planes, which are stained by anti-Flag and Alexa488-labelled secondary antibody.
- Supplemental movie 3 (.mpg, 670 KB) - Supplemental movie 3: fluorescence microscopy images (complete z-stack) of HaCaT cells co-transfected with EGFP-CReP and DSredER
- Supplemental movie 4 (.mpg, 311 KB) - Supplemental movie 4: fluorescence microscopy images (complete z-stack) of HaCaT cells co-transfected with EGFP-CReP(1-140) and DSredER.