800 primers pairs successfully resequenced in Pinaceae 727 successfully sequenced amplicons Agencourt Bioscience Corporation First check: duplicates and organella DNA 698 amplicons Identif_ication of potential gene function Second check: sequence quality 430 candidate genes 246 candidate genes 268 control genes 156 control genes 70 ABAL 61 LADE 171 PICE 190 PIMG 32 ABAL 35 LADE 108 PICE 120 PIMG