Cyclic Di-GMP Stimulates Biofilm Formation and Inhibits Virulence of Francisella novicida

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    Table S1. Bacterial strains and plasmids. Table S2. Primers used in this study. Fig. S1. Transposon insertion in cdgA does not adversely affect FTN0450 transcription. Fig. S2. Alignment of CdgA and CdgB with other DGCs (GGDEF-containing proteins). Fig. S3. Alignment of CdgA and CdgB with other PDEs (EAL domain-containing proteins). Fig. S4. Alignment of FTN0452 with CdgA, CdgB, and VCA0956. Fig. S5. CdgB stimulates V. cholerae biofilm formation. Fig. S6. Lack of CdgA is required for cdGMP-mediated inhibition of F. novicida intramacrophage replication. Fig. S7. Low cdGMP levels do not inhibit F. novicida virulence.

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