Mycobacterium tuberculosis CwsA Interacts with CrgA and Wag31, and the CrgA-CwsA Complex Is Involved in Peptidoglycan Synthesis and Cell Shape Determination

Supplemental material

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  Table S1, primers

  Fig. S1, localization of gfp-cwsA in M. tuberculosis

  Fig. S2, amino acid sequence alignment of cwsA homologues

  Fig. S3, morphology of M. smegmatis WT and ΔcwsA stationary-phase cultures

  Fig. S4, complementation of M. smegmatis ΔcwsA

  Fig. S5, ECFP-CrgA localization in M. smegmatis ΔcwsA

  Fig. S6, Wag31-mCherry can complement Δwag31

  Fig. S7, Wag31 levels do not change in a ΔcwsA strain

  Fig. S8, BACTH assays

  Fig. S9, confirmation of DKO strain by PCR

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