Broadly Neutralizing Antibodies Developed by an HIV-Positive Elite Neutralizer Exact a Replication Fitness Cost on the Contemporaneous Virus

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  Fig. S1 (Contribution of anti-CD4-BS NAbs to the overall neutralizing potential of plasma isolated from subject VC10042.)

  Fig. S2 (Numbers [log₁₀] of viral copies per milliliter of blood and of CD4⁺ and CD8⁺ T cells per microliter of blood during the time of observation.)

  Fig. S3 (Full-length gp160 amino acid alignment of the “early” clones isolated from plasma collected approximately 19 years postinfection.)

  Fig. S4 (Full-length gp160 amino acid alignment of the “late” clones isolated from plasma collected approximately 22 years postinfection.)

  Fig. S5 (Phylogenetic tree showing the relationship between the autologous env clones circulating in the plasma of subject VC10042 at 19 and 22 years postinfection and env clones from heterologous isolates tested for their susceptibility to neutralization by plasma from subject VC10042.)

  Fig. S6 (Regions on gp120 that mediate escape from the neutralizing activity of the anti-CD4-BS NAbs in plasma from subject VC10042.)

  Fig. S7 (Neutralization sensitivities of clone VC10042.y22.05 bearing reversion mutations to the monoclonal broadly neutralizing antibodies b12, VRC01, and NIH45-46^G54W.)

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