RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie01 - Movie 1. RhoD induces two types of thin protrusions. 10T1/2 cells transfected with EGFP--RhoD(G26V) were observed by time-lapse microscopy 48 h after the transfection.
  • Movie02 - Movie 2. Movement of fluorescent nodules through the RhoD-induced long and immotile protrusions. 10T1/2 cells transfected with EGFP--RhoD(G26V) were observed by time-lapse microscopy 48 h after the transfection.
  • Movie03 - Movie 3. Movement of FGFR1c- and Rab5-containing nodules through the RhoD--induced protrusions. 10T1/2 cells cotransfected with mOrange2--RhoD(G26V) (red), FGFR1c--EGFP (green), and Cerulean--Rab5(wt) (blue) were analyzed by time-lapse microscopy 48 h after the transfection.