Figure S1. Removal of wt genes from pAAV2_RaPID library.  The mixed population of parent (wt) and mutant genes was digested with NgoM IV, which is contained in the upstream sequence of the mCherry insert, but absent from the parent vector.  A bla gene (conferring resistance to carbenicillin) containing NgoM IV-compatible overhangs was ligated to the mixed vector pool.  Selection on agar supplemented with carbenicillin removed genes not containing an mCherry insert.  The bla cassette was then removed by digesting with NgoM IV, gel purification and vector religation.  The plasmid libraries used here contain the full AAV2 genomes, including rep and ITRs, but for simplicity are not illustrated here.