Files in this Data Supplement:
Movie 1. Ca2+ transients at late gastrula stage. Time-lapse movie of a late gastrula stage embryo (anterior is to the right) showing Ca2+ transients using the genetically encoded indicator GCaMP5 expressed in the neural plate. GCaMP5 was introduced by electroporation into the embryo at the 1-cell stage. For the embryo shown in the time-lapse, only one half (top) is expressing the transgene. The 20-second movie corresponds to 31.27 minutes of real time.
Movie 2. Ca2+ transients at neurula stage. Time-lapse movie of a mid-neurula stage embryo (anterior is to the right) showing Ca2+ transients using the genetically encoded indicator GCaMP5 expressed in the neural plate. The 14-second movie corresponds to 2.9 minutes of real time.
Movie 3. Addition of β-glycyrrhetinic acid blocks Ca2+ transients in the neural plate. In this embryo only the right-hand half of the neural plate is expressing the Ca2+ reporter (GCaMP5). The 11.2-second movie (not counting the text frames) corresponds to 7.7 minutes of real time.