Video 6.
ODN-activated PLTs do not form thrombi on collagen type IV. Human PRP was pretreated with 5 µM control ODN, type C CpG ODN, or a vehicle control and perfused at a shear rate of 200 s−1 (flow rate of 18.7 µl/min) over a surface coated with type IV collagen for 10 min. Although addition of ODN to PLTs resulted in PLT clumping in the presence of type IV collagen, these did not form thrombi as compared with type I collagen (positive control). Addition of type C CpG did not result in increased thrombus formation relative to the ODN control in PRP on the type I collagen-coated surface. Cells were viewed on an inverted microscope (TE-200) equipped with a 63× oil immersion objective, and images were obtained using a CCD camera (ORCA-ER).