Video 7.
ODN-activated PLTs do not form thrombi on collagen type IV. Human PRP was pretreated with 5 µM control ODN, type C CpG ODN, or a vehicle control and perfused at a shear rate of 200 s−1 (flow rate of 18.7 µl/min) over a surface coated with type I collagen for 10 min. Although addition of ODN to PLTs resulted in PLT clumping in the presence of type IV collagen, these did not form thrombi as compared with type I collagen (positive control). Addition of type C CpG did not result in increased thrombus formation relative to the ODN control in PRP on the type I collagen-coated surface. Cells were viewed on an inverted microscope (TE-200) equipped with a 63× oil immersion objective, and images were obtained using a CCD camera (ORCA-ER).