Video 2.
EFG-induced actin dynamics at junctional level of SH3BP1-depleted A431 cells. SH3BP1 siRNA-transfected, GFP-actin–expressing A431 cells were analyzed by time-lapse epifluorescence microscopy (Axiovert 200M). The focal plane was set to the junctional level. Images were taken every 5 s for 10 min and 35 s (frame rate of replay: 15 frames per second).