Video 3.
Actin cables migrated from the nonmedial region toward the middle during ring assembly in nmt1-wee1-50 adf1-1 LAGFP cells. Images were either acquired by time-lapse spinning-disk microscopy (right; Eclipse Ti microscope; microLAMBDA) or an optimized time-lapse spinning-disk microscopy (left; Axiovert 200M microscope) at 36°C. LAGFP is in black. Red asterisks show cables migrating from the nonmedial region to the cell middle. In both images, frames were collected every 30 s. Related to Fig. 2 D. Bars, 5 µm. For the whole video, frame rate is three frames per second.