Published November 26, 2012 // JCB vol. 199 no. 5 831-847
The Rockefeller University Press, doi: 10.1083/jcb.201209044

Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast

Video 5.
Nonmedial actin cables can also be observed in wt cells expressing Utr-CH-GFP mCh-Atb2p or Pact1-GFP-CHD. Images were either acquired by time-lapse spinning-disk microscopy (right; Eclipse Ti microscope; microLAMBDA) or an optimized time-lapse spinning-disk microscopy (left; Axiovert 200M microscope) at 24°C. (left) Related to Fig. 4 A. (right) Pact1-GFP-CHD is in black. Related to Fig. 4 E. In both panels, frames were collected every 25 s. For the whole video, frame rate is six frames per second.