Video 8.
Actin dynamics in for3Δ-related cells. Images were acquired by an optimized time-lapse spinning-disk microscopy (Axiovert 200M microscope) at 24°C. (left) for3Δ LAGFP mCh-Atb2 cell. Frames were collected every 25 s. Related to Fig. 6 A. (right) cdc25-22 for3Δ LAGFP mCh-Atb2p cell. Red arrows show a nonmedially assembled actin cable migrating toward the middle region during ring assembly. Frames were collected every 15 s. Related to Fig. 6 B. For the whole video, frame rate is five frames per second.