Published November 26, 2012 // JCB vol. 199 no. 5 831-847
The Rockefeller University Press, doi: 10.1083/jcb.201209044

Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast

Video 9.
Mitotic actin cables are either not prominent or not detectable during mitosis in cdc12Δ LAGFP mCh-Atb2p cell or for3Δ cdc12Δ LAGFP mCh-Atb2p cell. (left) cdc12Δ LAGFP mCh-Atb2p cell; (right) for3Δ cdc12Δ LAGFP mCh-Atb2p cell. Images were acquired by optimized time-lapse spinning-disk microscopy (Axiovert 200M microscope) at 24°C. In both images, cells were germinated from spores. In both images, frames were collected every 25 s. For the whole video, frame rate is 10 frames per second. Related to Fig. 6 (J–L).