Files in this data supplement:
- Supporting Information: Figures S1-S4, Files S1-S3, and Tables S1-S6 (PDF, 2.3 MB)
- Figure S1: The four baculoviral genes ie1, lef1, lef3 and p74 were initially cloned into pCRII TOPO vector (Invitrogen) (PDF, 1.8 MB)
- Figure S2: Copy number and site of insertion of transgenes in transgenic lines were characterized using TED assay (PDF, 144 KB)
- Figure S3: Mapping of transgenes to chromosome locations in different transgenic lines expressing dsRNA for essential baculoviral genes (PDF, 129 KB)
- Figure S4: Histogram representing percentage incorporation of the CSR2 genome in the BC4F6 population of transgenic lines, CSR2(118A), CSR2(164B) and CSR2(170B) obtained through recurrent backcross strategy, as measured by CSR2-specific SSR alleles from 10 loci (PDF, 84 KB)
- File S1: Additional Methods (PDF, 93 KB)
- File S2: Sequence of the selected viral genes (PDF, 81 KB)
- File S3: Genomic DNA sequences of different MG(+/-) transgenic lines surrounding piggyBac insertions, with the TTAA duplicated sequence appearing at all 5' and 3' insert boundaries (PDF, 78 KB)
- Table S1: PCR primers used for amplification of the different indicated genes/constructs (PDF, 66 KB)
- Table S2: List of transgenic lines obtained (PDF, 67 KB)
- Table S3: Location of insertion of transgenes (PDF, 66 KB)
- Table S4: Pairwise comparisons using Tukey HSD among groups on percentage mortality (values arcs in transformed) in transgenic lines, and their hybrids with CSR2 upon BmNPV treatment done after post-hoc analysis of the corresponding treatment factor using main effects in a one-way ANOVA (PDF, 78 KB)
- Table S5: Pairwise comparisons using Tukey HSD among groups on viral accumulation rate (values logarithm transformed) in transgenic and control lines upon BmNPV treatment was done after post-hoc analysis of the corresponding treatment factor using main effects in a one-way ANOVA (PDF, 71 KB)
- Table S6: List of 10 SSR loci with primer sequences, annealing temperature, MgCl2 concentration and corresponding amplicon size used to genotype the BC4F6 population (PDF, 75 KB)