Data from Macia et al. (2009) Figure 3B Hog1-PP time series for the Hog1as mutant (ste50Delta and ssk2Delta/ssk22Delta) without salt shock and 5 uM inhibitor treatment SD: standard deviation All Hog1 phosphorylation data are scaled to the wild type at 10 min after 0.4M NaCl shock time [min] ste50Delta SD ssk2/22Delta SD 0 1.5 2.09 2.09 0.61 10 5.59 2.08 2.08 1.64 20 20.03 2.29 2.29 0.49 30 31.68 1.96 1.96 0.77 40 38.94 2.03 2.03 0.64 50 38.88 1.79 1.79 1.77