Engineering Signal Peptides for Enhanced Protein Secretion from Lactococcus lactis

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Supplemental methods: mathematical model; nomenclature and parameter values used in the mathematical model (Table S1); bacterial strains and plasmids used in this study (Table S2); primers used in this study (Table S3); nucleotide sequences of selected clones from the Usp45sp silent mutation library (Table S4); nucleotide sequences of selected clones from the Usp45sp targeted mutation library (Table S5); nucleotide sequences of selected clones from the Usp45TM8 silent mutation library (Table S6); standard curve of α-amylase starch azure test (Fig. S1); nuclease plate test and α-amylase starch azure test of the two control signal peptides, Usp45sp and SP310 (Fig. S2).

  PDF, 182K