Files in this Data Supplement:
Effects of sulfate ion concentration, ions, and pH on TSD activity in cell-free extracts from A. ferrooxidans (Fig. S1); time course of absorbance changes at 420 nm after mixing thiosulfate and ferricyanide with the soluble fraction or the soluble fraction of A. ferrooxidans at pH 4 (Fig. S2); schematic map of the contig region containing the putative gene cluster context around gene tsd from A. ferrooxidans, and putative functions of AFEs present in this region (Fig. S3); transcriptional analysis of tsd gene in A. ferrooxidans ATCC 23270 by RT-PCR: schematic map of the genetic context around the tsd gene in A. ferrooxidans ATCC 23270, and agarose gel electrophoresis of DNA fragments amplified by RT-PCR (Fig. S4); multiple alignments of thiosulfate dehydrogenase homologues (Fig. S5) and of putative sulfate-binding proteins (Fig. S6); purification of recombinant TSD from soluble fraction of E. coli harboring pET-tsd (Table S1).
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